Analysis Note

ControlTested on Adult mouse brain lysate

Application

Research Sub CategoryNeurofilament & Neuron Metabolism

Detect NECL-1 using this Anti-NECL-1 Antibody validated for use in IH & WB.

Research CategoryNeuroscience

Western Blot Analysis: NECL-1 can be detected at 1/500 dilution. Testing at further dilution was not conducted.

Immunohistochemistry: 1:50 to 1:1000 straight dilution of Cat. # AB15301 was tested on human medulla oblongata tissue.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nectins are immunoglobulin superfamily adhesion molecules that participate in the organization of epithelial and endothelial junctions. Human and mouse NECL1 share 87.3% identity at the amino acid level (1). NECL1 contains an ectodomain made of three immunoglobulin-like domains, and a cytoplasmic region homologous to those of glycophorin C and contactin-associated protein. NECL1 predominantly expressed in the central nervous system, mainly in neuronal cell bodies in a variety of brain regions including the cerebellum, cerebral cortex and hippocampus. NECL1 localizes to the cell-cell junctions and recruits protein 4.1N to the plasma membranes through its C-terminus, thus may regulate the function of the cell-cell junction.

Immunogen

Synthetic Peptide

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified Antibody in PBS with 0.1% NaN3

ImmunoAffinity Purified

Specificity

NECL-1

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Target description

43 kDa